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      當前位置:主頁 > 技術文章 > 小鼠8羥基脫氧鳥苷,小鼠過氧化脂質ELISA試劑盒引用文獻

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      小鼠8羥基脫氧鳥苷,小鼠過氧化脂質ELISA試劑盒引用文獻
      時間:2022-02-15 點擊次數:152

      【文獻標題】Reparative effects of lycium barbarum polysaccharide on mouse ovarian injuries induced by repeated superovulation

      【作者】Bing Liu,Jun-Li Wang,Xin-Mei Wang,et.al

      【作者單位】北京農業大學(Beijing University of Agriculture)

      【文獻中引用產品】

      小鼠8羥基脫氧鳥苷(8-OHdG)ELISA試劑盒

      小鼠過氧化脂質(LPO)ELISA試劑盒

      【關鍵詞】Superovulatory times,Injury model,LBP,Remedy,Oxidative stress

      【DOI】doi.org/10.1016/j.theriogenology.2020.01.048

      【出版期刊】《Theriogenology》

      【產品原文引用】

      8-OHdG and LPO levels of the collected ovarian tissues were determined using an ELISA kit, according to the manu-facturer’s instructions (Shanghai Hengyuan Biotechnology Co., Ltd,Shanghai, China). The collected mouse ovaries were cracked with lysate. Each 10 mL henylmethane-sulfonyl (PMSF) was added in 1 mL radio immuno precipitation assay (RIPA) as lysate. Every 1 g of tissue with 9 mL lysate, then coated with mouse antibody against 8-OHdG or LPO. After incubation and washing concentrations of 8-OHdG or LPO were determined as described previously .Intra-and inter-CVs: 8-OHdG (6.3%, 8.1%), LPO (5.2%, 7.4%).



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